Deteksi Variasi Somaklonal Planlet Jatropha Curcas Linn. Hasil Regenerasi Embrio Somatik Dengan Marka Molekular ISSR
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Deteksi Variasi Somaklonal Planlet Jatropha Curcas Linn. Hasil Regenerasi Embrio Somatik Dengan Marka Molekular ISSR
- Publication date
- 2021-08-01
- Usage
- Public Domain Mark 1.0
- Topics
- 2, 4-D, ISSR, J. curcas, proliferation, somatic embryos
- Collection
- opensource
- Language
- Indonesian
- Item Size
- 9.6M
Physic nut (Jatropha curcas Linn.) has the potential as a source of sustainable biofuels. Somatic embryo proliferation of J. curcas may cause somaclonal variations. This research aimed to investigate somaclonal variations of J. curcas somatic embryo derived-plantlet using ISSR markers. Somatic embryos of J. curcas at the globular phase were cultured on liquid MS medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg L-1 of 2,4-D. Parameter observed were embryos weight, embryos volume, colour, and size of embryos. After proliferation, the embryoswere cultured on a germination medium until the cotyledonary phase. The plantlet’s leaveswere used for DNA samples for ISSR analysis. The results showed that proliferation of J. curcas somatic embryos was optimal at MS medium supplemented with 1 mg L-1 2,4-D and produced the highest weight and volume of embryos. The furthest genetic distance occurred between the control and J. curcas plantlet which was regenerated from MS + 1 mg L-1 2,4-D which had 0.60 of similarity coefficient.
- Addeddate
- 2024-08-06 01:37:19
- Identifier
- deteksi-variasi-somaklonal-planlet-jatropha-curcas-linn.-hasil-regenerasi-embrio
- Identifier-ark
- ark:/13960/s2sq40xjnft
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- Latin
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- 0.0.21
- Ocr_parameters
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- Page_number_confidence
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- Page_number_module_version
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- Ppi
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- Scanner
- Internet Archive HTML5 Uploader 1.7.0
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